Vista Equipo: Using NextRAD sequencing to infer movement of herbivores among host plants

Using NextRAD sequencing to infer movement of herbivores among host plants

Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insuffic...

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Autores Principales:	Fu, Zhen, Epstein, Brendan, Kelley, Joanna L., Zheng, Qi, Bergland, Alan O., Castillo C., Carmen, Jensen, Andrew S., Dahan, Jennifer, Karasev, Alexander V., Snyder, William E.
Formato:	Artículos
Lenguaje:	en
Publicado:	2017
Materias:	SOLANUM DULCAMARA PLAGAS BACTERICERA COCKERELLI HOSPEDEROS ALTERNOS NGS SECUENCIACIÓN
Acceso en línea:	http://repositorio.iniap.gob.ec/handle/41000/4508
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spelling	ir-:41000-45082018-04-12T19:23:08Z Using NextRAD sequencing to infer movement of herbivores among host plants Plos One 12(5):1-19 Fu, Zhen Epstein, Brendan Kelley, Joanna L. Zheng, Qi Bergland, Alan O. Castillo C., Carmen Jensen, Andrew S. Dahan, Jennifer Karasev, Alexander V. Snyder, William E. SOLANUM DULCAMARA PLAGAS BACTERICERA COCKERELLI HOSPEDEROS ALTERNOS NGS SECUENCIACIÓN Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insufficient genetic resolution to infer spread at finer spatiotemporal scales. Here, we explore the use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer movement of a highly-mobile winged insect, the potato psyllid (Bactericera cockerelli), among host plants. The psyllid vectors the pathogen that causes zebra chip disease in potato (Solanum tuberosum), but understanding and managing the spread of this pathogen is limited by uncertainty about the insect’s host plant(s) outside of the growing season. We identified 1,978 polymorphic loci among psyllids separated spatiotemporally on potato or in patches of bittersweet nightshade (S. dulcumara), a weedy plant proposed to be the source of potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic similarity to insects on nightshade, consistent with regular movement between these two host plants. However, a second subset of potato-collected psyllids was genetically distinct from those collected on bittersweet nightshade; this suggests that a currently unrecognized source, i.e., other nightshade patches or a third host-plant species, could be contributing to psyllid populations in potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine scale in order to understand, predict, and manage disease spread. We demonstrate that emerging sequencing technologies that detect genome-wide SNPs of a vector can be used to infer such localized movement. 2017-07-19T18:34:40Z 2017-07-19T18:34:40Z 2017-05-15 Revista Artículo *EC-INIAP-BEESC-MGC. Quito (INIAP/CD192CC) http://repositorio.iniap.gob.ec/handle/41000/4508 en p. 1-19 application/pdf E. E. Santa Catalina
institution	INIAP
collection	Repositorio INIAP
biblioteca	Biblioteca INIAP
language	en
format	Artículos
topic	SOLANUM DULCAMARA PLAGAS BACTERICERA COCKERELLI HOSPEDEROS ALTERNOS NGS SECUENCIACIÓN
spellingShingle	SOLANUM DULCAMARA PLAGAS BACTERICERA COCKERELLI HOSPEDEROS ALTERNOS NGS SECUENCIACIÓN Fu, Zhen Epstein, Brendan Kelley, Joanna L. Zheng, Qi Bergland, Alan O. Castillo C., Carmen Jensen, Andrew S. Dahan, Jennifer Karasev, Alexander V. Snyder, William E. Using NextRAD sequencing to infer movement of herbivores among host plants
description	Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insufficient genetic resolution to infer spread at finer spatiotemporal scales. Here, we explore the use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer movement of a highly-mobile winged insect, the potato psyllid (Bactericera cockerelli), among host plants. The psyllid vectors the pathogen that causes zebra chip disease in potato (Solanum tuberosum), but understanding and managing the spread of this pathogen is limited by uncertainty about the insect’s host plant(s) outside of the growing season. We identified 1,978 polymorphic loci among psyllids separated spatiotemporally on potato or in patches of bittersweet nightshade (S. dulcumara), a weedy plant proposed to be the source of potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic similarity to insects on nightshade, consistent with regular movement between these two host plants. However, a second subset of potato-collected psyllids was genetically distinct from those collected on bittersweet nightshade; this suggests that a currently unrecognized source, i.e., other nightshade patches or a third host-plant species, could be contributing to psyllid populations in potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine scale in order to understand, predict, and manage disease spread. We demonstrate that emerging sequencing technologies that detect genome-wide SNPs of a vector can be used to infer such localized movement.
author	Fu, Zhen Epstein, Brendan Kelley, Joanna L. Zheng, Qi Bergland, Alan O. Castillo C., Carmen Jensen, Andrew S. Dahan, Jennifer Karasev, Alexander V. Snyder, William E.
author_facet	Fu, Zhen Epstein, Brendan Kelley, Joanna L. Zheng, Qi Bergland, Alan O. Castillo C., Carmen Jensen, Andrew S. Dahan, Jennifer Karasev, Alexander V. Snyder, William E.
author_sort	Fu, Zhen
title	Using NextRAD sequencing to infer movement of herbivores among host plants
title_short	Using NextRAD sequencing to infer movement of herbivores among host plants
title_full	Using NextRAD sequencing to infer movement of herbivores among host plants
title_fullStr	Using NextRAD sequencing to infer movement of herbivores among host plants
title_full_unstemmed	Using NextRAD sequencing to infer movement of herbivores among host plants
title_sort	using nextrad sequencing to infer movement of herbivores among host plants
publishDate	2017
url	http://repositorio.iniap.gob.ec/handle/41000/4508
_version_	1635511995829583872
score	11,871979

Using NextRAD sequencing to infer movement of herbivores among host plants

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