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Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India

To study the variability and to identify the species of Begomovirus associated with yellow mosaic disease of blackgram in Andhra Pradesh, India, infected blackgram samples were collected from six districts belonging to three regions of Andhra Pradesh. The total DNA was isolated by modified CTAB meth...

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Autor Principal: Yeturu, Siva Prasad
Formato: Artículos
Lenguaje:eng
Publicado: 2017
Materias:
VIGNA MUNGO
BEGOMOVIRUS
PCR
COAT PROTEIN
Acceso en línea:http://repositorio.educacionsuperior.gob.ec/handle/28000/4070
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spelling oai:localhost:28000-40702017-04-17T16:10:57Z Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India Yeturu, Siva Prasad VIGNA MUNGO BEGOMOVIRUS PCR COAT PROTEIN To study the variability and to identify the species of Begomovirus associated with yellow mosaic disease of blackgram in Andhra Pradesh, India, infected blackgram samples were collected from six districts belonging to three regions of Andhra Pradesh. The total DNA was isolated by modified CTAB method and amplified with coat protein gene-specific primers (RHA-F and AC abut) resulting in 900?bp gene product. The PCR products were cloned, sequenced and deposited in GenBank. The sequence analysis of six clones showed that the size of amplified CP gene of YMV was 920?bp. Based on nucleotide sequence identity of six isolates representing three regions of Andhra Pradesh, the isolates from Rayalaseema and Telangana region are the same variant of YMV (>99.5% identity) and isolate from coastal Andhra is another variant of YMV (>95.4%) when compared with other region isolates. Comparison of CP gene sequence of YMV-TPT isolate with 27 other isolates in database revealed more than 93.2 and 86.2% identity with MYMIV isolates and less than 80 and 64% identity with MYMY isolates that originate from Indian sub-continent and South-East Asia at nucleotide and amino acid level, respectively. Phylogenetic tree based on CP gene sequences of six isolates with other isolates from GenBank formed unique cluster with MYMIV. Hence the YMV infecting blackgram in Andhra Pradesh is caused by MYMIV rather than MYMY as reported in Tamil Nadu which is adjoining state in southern India. https://www.researchgate.net/publication/269775438_Mungbean_yellow_m osaic_India_virus_is_associated_with_yellow_mosaic_disease_of_blackgra m_Vigna_mungo_L_in_Andhra_Pradesh_India 2017-04-12T18:59:36Z 2017-04-12T18:59:36Z 2014 article Bhaskara Reddy, BV. et al. (2014). Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India. Archives of Phytopathology and Plant Protection. Vol. 48. Issue 4. Reino Unido. pp. 345-353 http://repositorio.educacionsuperior.gob.ec/handle/28000/4070 eng closedAccess http://creativecommons.org/licenses/by-nc-sa/3.0/ec/
institution SENESCYT
collection Repositorio SENESCYT
biblioteca Biblioteca Senescyt
language eng
format Artículos
topic VIGNA MUNGO
BEGOMOVIRUS
PCR
COAT PROTEIN
spellingShingle VIGNA MUNGO
BEGOMOVIRUS
PCR
COAT PROTEIN
Yeturu, Siva Prasad
Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
description To study the variability and to identify the species of Begomovirus associated with yellow mosaic disease of blackgram in Andhra Pradesh, India, infected blackgram samples were collected from six districts belonging to three regions of Andhra Pradesh. The total DNA was isolated by modified CTAB method and amplified with coat protein gene-specific primers (RHA-F and AC abut) resulting in 900?bp gene product. The PCR products were cloned, sequenced and deposited in GenBank. The sequence analysis of six clones showed that the size of amplified CP gene of YMV was 920?bp. Based on nucleotide sequence identity of six isolates representing three regions of Andhra Pradesh, the isolates from Rayalaseema and Telangana region are the same variant of YMV (>99.5% identity) and isolate from coastal Andhra is another variant of YMV (>95.4%) when compared with other region isolates. Comparison of CP gene sequence of YMV-TPT isolate with 27 other isolates in database revealed more than 93.2 and 86.2% identity with MYMIV isolates and less than 80 and 64% identity with MYMY isolates that originate from Indian sub-continent and South-East Asia at nucleotide and amino acid level, respectively. Phylogenetic tree based on CP gene sequences of six isolates with other isolates from GenBank formed unique cluster with MYMIV. Hence the YMV infecting blackgram in Andhra Pradesh is caused by MYMIV rather than MYMY as reported in Tamil Nadu which is adjoining state in southern India.
author Yeturu, Siva Prasad
author_facet Yeturu, Siva Prasad
author_sort Yeturu, Siva Prasad
title Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
title_short Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
title_full Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
title_fullStr Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
title_full_unstemmed Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India
title_sort mungbean yellow mosaic india virus is associated with yellow mosaic disease of blackgram (vigna mungo l.) in andhra pradesh, india
publishDate 2017
url http://repositorio.educacionsuperior.gob.ec/handle/28000/4070
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score 11,871979

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