Imagen de Portada

Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees

To improve the potential of tamarind as an economically valued domesticated species it is important to characterize its variability in Ecuador for breeding purposes. Our aim was to investigate the genetic diversity of 32 tamarind plus trees using inter-simple sequence repeat (ISSR) markers. Eighty f...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores Principales: Sarmiento Calva, Leidy, Pérez-Almeida, Iris, Díaz, Byron, Alvarez, Hugo, Viera, William
Formato: Artículos
Lenguaje:en
Publicado: 2018
Materias:
TAMARINDO
TAMARINDUS INDICA
MEJORAMIENTO DE FRUTALES
MARCADORES MOLECULARES
Acceso en línea:http://repositorio.iniap.gob.ec/handle/41000/5076
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id ir-:41000-5076
recordtype dspace
institution INIAP
collection Repositorio INIAP
biblioteca Biblioteca INIAP
language en
format Artículos
topic TAMARINDO
TAMARINDUS INDICA
MEJORAMIENTO DE FRUTALES
MARCADORES MOLECULARES
spellingShingle TAMARINDO
TAMARINDUS INDICA
MEJORAMIENTO DE FRUTALES
MARCADORES MOLECULARES
Sarmiento Calva, Leidy
Pérez-Almeida, Iris
Díaz, Byron
Alvarez, Hugo
Viera, William
Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
description To improve the potential of tamarind as an economically valued domesticated species it is important to characterize its variability in Ecuador for breeding purposes. Our aim was to investigate the genetic diversity of 32 tamarind plus trees using inter-simple sequence repeat (ISSR) markers. Eighty four loci were examined using 12 markers, with a mean number of 4.42 loci per primer; 8 loci (9.52 %) were monomorphic and 76 (90.48 %) polymorphic, revealing genetic variability among the individuals. Polymorphic information content (PIC) values varied from 0.29 (ISSR_808) to 0.93 (ISSR_HB12), whereas the marker index ranged from to 26.4 (ISSR_814) to 62.5 (ISSR_17899A). Primers ISSR_HB11, ISSR_836, ISSR_842, ISSR_848, ISSR_860, ISSR_17899A and ISSR_17899B were useful to discriminate the grouping of the accessions according to their PIC values. Ward cluster analysis grouped accessions into two major groups with five subgroups with 46 % similarity according to Jaccard distance. The genotypes from Loja, Manabí and Guayas provinces were grouped in the first cluster; while only individuals from Manabí located in the other group, indicating major diversity in the latter province. Genotypes T1-ECUM-001 and T1-ECUM-002 presented 76 % similarity, while T1-ECUM-008, T1-ECUM-010, T1-ECUM-012, T1-ECUM-017 and T1-ECUM-018 shared 60 %. All materials from Loja grouped with 65 % similarity. Other genotypes clustered with similarity of 54 %. The cophenetic correlation coefficient (0.634) showed a good fit between the data matrix and the dendrogram results. A reasonable degree of diversity was found among tamarind genotypes potentially useful to select plus trees for clonal propagation as well as to identify diverse parents for hybridization programs.
author Sarmiento Calva, Leidy
Pérez-Almeida, Iris
Díaz, Byron
Alvarez, Hugo
Viera, William
author_facet Sarmiento Calva, Leidy
Pérez-Almeida, Iris
Díaz, Byron
Alvarez, Hugo
Viera, William
author_sort Sarmiento Calva, Leidy
title Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
title_short Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
title_full Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
title_fullStr Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
title_full_unstemmed Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
title_sort molecular marker-based characterization of ecuadorian dry forest tamarind plus trees
publishDate 2018
url http://repositorio.iniap.gob.ec/handle/41000/5076
_version_ 1635512147901415424
spelling ir-:41000-50762018-08-14T17:16:29Z Molecular marker-based characterization of ecuadorian dry forest tamarind plus trees Caracterización molecular de árboles élite de tamarindo del bosque seco ecuatoriano Biagro 29(3):153-162 Sarmiento Calva, Leidy Pérez-Almeida, Iris Díaz, Byron Alvarez, Hugo Viera, William TAMARINDO TAMARINDUS INDICA MEJORAMIENTO DE FRUTALES MARCADORES MOLECULARES To improve the potential of tamarind as an economically valued domesticated species it is important to characterize its variability in Ecuador for breeding purposes. Our aim was to investigate the genetic diversity of 32 tamarind plus trees using inter-simple sequence repeat (ISSR) markers. Eighty four loci were examined using 12 markers, with a mean number of 4.42 loci per primer; 8 loci (9.52 %) were monomorphic and 76 (90.48 %) polymorphic, revealing genetic variability among the individuals. Polymorphic information content (PIC) values varied from 0.29 (ISSR_808) to 0.93 (ISSR_HB12), whereas the marker index ranged from to 26.4 (ISSR_814) to 62.5 (ISSR_17899A). Primers ISSR_HB11, ISSR_836, ISSR_842, ISSR_848, ISSR_860, ISSR_17899A and ISSR_17899B were useful to discriminate the grouping of the accessions according to their PIC values. Ward cluster analysis grouped accessions into two major groups with five subgroups with 46 % similarity according to Jaccard distance. The genotypes from Loja, Manabí and Guayas provinces were grouped in the first cluster; while only individuals from Manabí located in the other group, indicating major diversity in the latter province. Genotypes T1-ECUM-001 and T1-ECUM-002 presented 76 % similarity, while T1-ECUM-008, T1-ECUM-010, T1-ECUM-012, T1-ECUM-017 and T1-ECUM-018 shared 60 %. All materials from Loja grouped with 65 % similarity. Other genotypes clustered with similarity of 54 %. The cophenetic correlation coefficient (0.634) showed a good fit between the data matrix and the dendrogram results. A reasonable degree of diversity was found among tamarind genotypes potentially useful to select plus trees for clonal propagation as well as to identify diverse parents for hybridization programs. Para aumentar el potencial del tamarindo como especie domesticada con valor económico es importante caracterizar la variabilidad en Ecuador con propósitos de mejoramiento. Nuestro objetivo fue investigar la diversidad genética de 32 árboles élite de tamarindo utilizando marcadores de secuencia inter-simple repetida (ISSR). Se examinaron 84 loci con 12 marcadores, con un número promedio de 4,42 loci per primer; 8 loci (9,52 %) fueron monomórficos y 76 (90,48 %) polimórficos, revelando variabilidad genética entre individuos. El contenido de información polimórfica (PIC) osciló entre 0,29 (ISSR_808) y 0,93 (ISSR_HB12), mientras que el índice de marcador fluctuó entre 26,4 (ISSR_814) y 62,5 (ISSR_17899A). ISSR_HB11, ISSR_836, ISSR_842, ISSR_848, ISSR_860, ISSR_17899A e ISSR_17899B fueron útiles para discriminar accesiones según sus CIPs. El análisis de conglomerados de Ward formó dos grupos principales y cinco subgrupos con 46 % de similitud según la distancia de Jaccard. Genotipos de Loja, Manabí y Guayas se aglomeraron en un grupo; mientras que sólo accesiones de Manabí quedaron en el otro, indicando mayor diversidad en la última provincia. Los genotipos T1-ECUM-001 y T1-ECUM-002 presentaron 76 % similitud, mientras T1-ECUM-008, T1-ECUM-010, T1-ECUM-012, T1-ECUM-017 y T1-ECUM-018 compartieron 60 %. Todos los materiales de Loja se agruparon con 65 % de similitud. Otros genotipos se concentraron con similitud de 54 %. El coeficiente de correlación cofenética (0,634) mostró buen ajuste entre la matriz de datos y los resultados del dendrograma. Se encontró un grado razonable de diversidad entre los genotipos de tamarindo potencialmente útil para seleccionar árboles élite para propagación clonal así como para identificar progenitores diversos para programas de hibridación. 2018-08-07T16:06:04Z 2018-08-07T16:06:04Z 2017-05 Revista Artículo 1316-3361 *EC-INIAP-BEEP-LA. Portoviejo (Biagro 29(3):153-162. 2017) http://repositorio.iniap.gob.ec/handle/41000/5076 en p. 153-162 application/pdf E. E. Portoviejo
score 11,871979

Ejemplares similares