Vista Equipo: Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum

Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum

Centrolobium ochroxylum, a forest species is of great commercial value. Indirect somatic embryogenesis has been developed from leaf explants for Centrolobium ochroxylum. Seven different media were tested for the induction. Embryogenic callus was observed using half strength MS medium supplemented wi...

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Autores Principales:	Quiroga Quisaguano, Santiago Andrés, Morillo, Eduardo, Orbe, Katerine, Meneses Montesdeoca, Luis Santiago, Buitrón Bustamante, Johanna Liseth
Formato:	Artículos
Lenguaje:	en
Publicado:	2017
Materias:	CENTROLOBIUM OCHROZYLUM EMBRIOGÉNESIS SOMÁTICA
Acceso en línea:	http://repositorio.iniap.gob.ec/handle/41000/4106
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id	ir-:41000-4106
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spelling	ir-:41000-41062018-03-26T16:06:32Z Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum International Journal of Clinical and Biological Sciences 1(1):1-10 Quiroga Quisaguano, Santiago Andrés Morillo, Eduardo Orbe, Katerine Meneses Montesdeoca, Luis Santiago Buitrón Bustamante, Johanna Liseth CENTROLOBIUM OCHROZYLUM EMBRIOGÉNESIS SOMÁTICA Centrolobium ochroxylum, a forest species is of great commercial value. Indirect somatic embryogenesis has been developed from leaf explants for Centrolobium ochroxylum. Seven different media were tested for the induction. Embryogenic callus was observed using half strength MS medium supplemented with different concentrations of picloram under complete dark conditions. Medium supplemented with 5 mg/l picloram showed the highest percentage (68%) of embryogenic callus. The histological observation revealed the presence of isodiametric cells with a prominent nucleus at the centre. Development of somatic embryos was also observed in another three media without plant growth regulators. Starch grains and storage proteins were observed in matured somatic embryos. This is the first report on somatic embryogenesis in Centrolobium ochroxylum. 2017-03-15T21:04:05Z 2017-03-15T21:04:05Z 2016-01 Revista Artículo *EC-INIAP-BEESC-MGC. Quito (INIAP/CD105) http://repositorio.iniap.gob.ec/handle/41000/4106 en p. 1-10 application/pdf E. E. Santa Catalina
institution	INIAP
collection	Repositorio INIAP
biblioteca	Biblioteca INIAP
language	en
format	Artículos
topic	CENTROLOBIUM OCHROZYLUM EMBRIOGÉNESIS SOMÁTICA
spellingShingle	CENTROLOBIUM OCHROZYLUM EMBRIOGÉNESIS SOMÁTICA Quiroga Quisaguano, Santiago Andrés Morillo, Eduardo Orbe, Katerine Meneses Montesdeoca, Luis Santiago Buitrón Bustamante, Johanna Liseth Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
description	Centrolobium ochroxylum, a forest species is of great commercial value. Indirect somatic embryogenesis has been developed from leaf explants for Centrolobium ochroxylum. Seven different media were tested for the induction. Embryogenic callus was observed using half strength MS medium supplemented with different concentrations of picloram under complete dark conditions. Medium supplemented with 5 mg/l picloram showed the highest percentage (68%) of embryogenic callus. The histological observation revealed the presence of isodiametric cells with a prominent nucleus at the centre. Development of somatic embryos was also observed in another three media without plant growth regulators. Starch grains and storage proteins were observed in matured somatic embryos. This is the first report on somatic embryogenesis in Centrolobium ochroxylum.
author	Quiroga Quisaguano, Santiago Andrés Morillo, Eduardo Orbe, Katerine Meneses Montesdeoca, Luis Santiago Buitrón Bustamante, Johanna Liseth
author_facet	Quiroga Quisaguano, Santiago Andrés Morillo, Eduardo Orbe, Katerine Meneses Montesdeoca, Luis Santiago Buitrón Bustamante, Johanna Liseth
author_sort	Quiroga Quisaguano, Santiago Andrés
title	Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
title_short	Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
title_full	Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
title_fullStr	Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
title_full_unstemmed	Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum
title_sort	morphological and histological ontogeny of somatic embryogenesis in centrolobium ochroxylum
publishDate	2017
url	http://repositorio.iniap.gob.ec/handle/41000/4106
_version_	1635512289824079872
score	11,871979

Morphological and histological ontogeny of Somatic embryogenesis in Centrolobium ochroxylum

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